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restriction endonucleases, in DNA sequences. Double Digest Finder Use this tool to guide your reaction buffer selection when setting up double-digests, a common timesaving procedure.
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Otherwise, choose an NEBuffer that results in the most activity for both enzymes. Double Digest Finder Use this tool to guide your reaction buffer selection when setting up double-digests, a common timesaving procedure. Choosing the right buffers will help you to avoid star activity and loss of product.
The resulting fragments are sorted by size, and they are given a title specifying their length, their position in the original sequence, and the enzyme sites that produced them.
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Simply write any calculation as you would on paper.